- Sandwich Shop Restaurants, Chiefland
- Satellite & Cable TV Equipment & Systems Repair & Service, Chiefland
- Satellite Communication Downlinking & Uplinking, Chiefland
- Satellite TV Service, Chiefland
- Scrap & Waste Materials, Chiefland
- Scrapbooks & Scrapbook Supplies, Chiefland
- Secondary Schools, Chiefland
- Security Brokers & Dealers, Chiefland
- Senior Citizens Service & Health Organizations, Chiefland
- Septic Tank Installation & Repair, Chiefland - Septic Systems
- Septic Tank Pumping & Cleaning, Chiefland - Septic Systems
- Septic Tanks & Septic Systems Contractors, Chiefland - Installation, Repair, Service, Cleaning & Pumping Septic Tanks
- Shoe Dyers, Chiefland
- Shoe Repair Shops, Chiefland
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- Shoe Stores, Chiefland - Mens, Womens & Kids Shoes
- Shopping & Shopping Services, Chiefland
- Signs, Chiefland
- Sleep Disorders Treatment & Information Centers, Chiefland
- Social Services & Welfare, Chiefland
- Societies & Foundation Associations, Chiefland
- Special Education & Care, Chiefland
- Specialty Animal Services, Chiefland
- Specialty Stores, Chiefland
- Sporting Goods Stores, Chiefland
- Sports Bars & Lounges, Chiefland
- State Government, Chiefland
- Stocks & Bond Brokers, Chiefland
- Swimming Pools Supplies, Equipment & Accessories, Chiefland
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